Thy1-gcamp6 transgenic mice
Webb11 okt. 2024 · Transgenic mice expressing the green GECI GCaMP6 are already widely used. Here we present the generation and characterization of transgenic mice … Webb20 jan. 2024 · Transgenic Thy1.2-GCaMP6s mice expressing GCaMP6 slow in some DRG neurons have been previously described (61). As no significant differences were observed between male and female mice,...
Thy1-gcamp6 transgenic mice
Did you know?
Webb16 feb. 2024 · Thy1-GCaMP6s transgenic mice have been developed by transgenic meth- ods and express GCaMP6s under the Thy1promoter, in which projection neurons in the cerebral cortex and hippocampus... Webb18 okt. 2012 · To generate GCaMP transgenic mice, we used the well-characterized Thy1 promoter to express GCaMPs in neurons. We generated eight founder lines of …
WebbVariants of GCaMP fluoresce in response to calcium binding and serve as an indicator of neuronal activation. These include Thy1-promoter driven GCaMP6 transgenic lines, Tet-dependent GCaMP6f or GCaMP6s transgenic lines and Cre-dependent GCaMP3, GCaMP5, GCaMP6f or GCaMP6s mouse lines. Webb23 juli 2024 · For example, Thy1-GCaMP6 mice show stable expression of GCaMP6 in multiple cortical regions across months without apparent toxicity, but only after the third postnatal week (Dana et al., 2014). Similarly, the Ai38 mouse line expresses GCaMP3 when treated with tamoxifen at P7, but showed very low expression at 4 weeks of age …
Webb7 dec. 2012 · Chen et al. report transgenic lines expressing either GCaMP2.2c or GCaMP3 under the control of the Thy1 promoter. These mice showed GCaMP fluorescence in … Webb29 jan. 2015 · Sanford Burnham Prebys. Nov 2024 - Present6 months. La Jolla, CA. Management of laboratory operations and support drug …
Webb024276 C57BL/6J-Tg(Thy1-GCaMP6f)GP5.5Dkim/J These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector ( GENIE ) Project, express the green …
Webb24 sep. 2014 · Thy1 -GCaMP6-WPRE transgenic mice were generated using standard techniques [23] .We included the WPRE (Woodchuck hepatitis virus post-transcriptional … someonevwearing oversized glassesWebb1 jan. 2012 · It is shown that GCaMP6 Thy1 transgenic mice are useful for long-term, high-sensitivity imaging in behaving mice and are shown to be effective for in vivo imaging of genetically defined neuronal populations in vivo. 407 PDF View 2 excerpts, cites background DEVELOPMENT OF A NONINVASIVE CALCIUM IMAGING PROBE small cabin plans and costWebbFurthermore, we show that GCaMP6 Thy1 transgenic mice are useful for long-term, high-sensitivity imaging in behaving mice. View on publisher site Alert me about new mentions. Twitter Demographics. The data shown below were collected from the profiles of 2 tweeters who shared this research output. someone waiting in the skyWebb23 sep. 2024 · Adult (P65-P70) transgenic mice (C57BL/6J-Tg (Thy1-GCaMP6f) GP5.1Dkim/J; The Jackson Laboratory) expressing GCaMP6f under the Thy1 promoter were used. All animals were housed in a temperature-controlled (24 °C) colony room and maintained in a 12-h/12-h light/dark cycle (lights on at 7:00 a.m.). Food and water were … small cabin like homesWebb24 sep. 2014 · We quantified GCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated … small cabin kits texasWebb6 apr. 2024 · We had previously shown that THY1 (CD90) is a tumor suppressor in nasopharyngeal carcinoma (NPC) and that its down-regulation and loss of expression are associated with tumor metastasis, yet the mechanism leading to such effects remains unknown. In this study we show that tumor invasion could be suppressed by THY1 via … small cabin minecraft ideasWebbThy1 -GCaMP6-WPRE transgenic mice were generated using standard techniques [23].We included the WPRE (Woodchuck hepatitis virus post-transcriptional regulatory element), … small cabin prefab maryland