WebJul 5, 2024 · To maximize data obtainment from valuable cardiac tissue, we hypothesized that myocardium fixed in optimal cutting temperature (OCT) medium for histology could also be used to investigate the function of myofilament proteins in situ. We compared tissue prepared via conventional liquid nitrogen (LN) snap freezing with tissue fixed in OCT and … Web1. Snap freeze the tissue and store it in a sealed container at -80C. Embed tissue in OCT before cryosectioning. Prepare an isopentane and liquid nitrogen bath. Place tissue using …
Tissue Embedding Fisher Scientific
Web6. Record the appropriate information about the tissue in your tissue repository. NOTE: Records should include all of the following: tissue identity, date frozen, freezing medium used, and method and results of all quality control tests performed. Sample Warming 1. Place the Corning® CoolRack® module into a 37°C water bath. Alternatively, a ... WebDescription Description Yield high-quality sections down to -50°C without chatter using Epredia; Neg-50™ Frozen Section Medium, a water-soluble frozen section medium. Buffered to ensure proper alkalinity (pH = 8.0); … inceptionv3模型下载
PolyFreeze Tissue Freezing Medium clear Sigma-Aldrich
WebProtocol for dry ice/isopentane freezing 1. Place the container with isopentane onto dry ice 15–20 min before embedding so the isopentane becomes cold. 2. Dissect the tissue (approximately 1×1 cm). 3. Incubate the tissue in optimal cutting temperature (OCT) compound at room temperature (2 to 3 changes, 1 min each). 4. Add OCT into plastic … WebOCT (Optical Cutting Temperature) (Fisher Scientific, Cat#4583) or NEG50TM (Richard-Allan Scientific, Cat#6502), minimizing bubbles. 3. Wick off excess surrounding liquid from the … Web1. Snap freeze the tissue and store it in a sealed container at -80C. Embed tissue in OCT before cryosectioning. Prepare an isopentane and liquid nitrogen bath. Place tissue using forceps or spatula into isopentane until completely frozen ~1 min depending upon tissue size. Transfer tissue into pre-chilled container on dry-ice. inceptionv3 pytorch实现